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Subject: PSDN-1609 SCIENCE SITREP 
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PSDN-1609 SCIENCE SITREP 
R 040109Z SEP 93
FROM: Thomas K. Frazer, Science Leader


       P A L M E R   S T A T I O N   A N T A R C T I C A
   TELEMAIL::PALMER.STA          PHONE/FAX:  011-874-150-3157
       SPAN::PALMER@ATSVAX.SPAN      TELEX:  5841503157 PNHG
   INTERNET::PALMER@ATSVAX.RSMAS.MIAMI.EDU


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S-010     Ozone Dimunition, Ultraviolet Radiation and Phytoplankton
          Biology in  Antarctic Waters.  Co-Principal
          Investigators:  B. Prezelin (S-010) and R. Smith (S-034).

          Field Team (S-010):  B. Prezelin, N. Boucher, T. Evens,
          R. Jovine, B. Kroon, M. Moline and O. Schofield 

     Our objective for our 3 month stay in Antarctica (August 8th-
November 8th) is to quantify the UV dependency of production, 
photodamage & photoprotective responses in diverse Antarctic 
phytoplankton communities.  Specifically, we aim to repeat previous
in situ mooring studies in the MIZ and determine UV inhibition
effects on key target sites known to occur in phytoplankton.
Target sites for the proposed study include:

          i) DNA  photodamage,
          ii) Photosynthetic electron flow and
          iii) Photoprotective carotenoids & mycosporine-like amino
               acids.

We also planto return with 'time capsules' of microbial DNA that
will be available for us & other researchers to examine for       
possible genetic effects of UV radiation on Antarctic marine
microbial communities.

     Ice coverage has been highly variable since field exercises
began  August 17th, thus enabling us to conduct studies in frazil
ice, water column and benthic communities of phytoplankton. Working 
nearshore waters around Palmer, we collected thus far collected 25
HPLC pigment, nutrient and CHN samples, 15 MAA samples, and 10
large volume samples of microbial DNA. In addition we have 
measured photosynthesis-irradiance curves (using Qpar-
photosynthetrons) for freshly collected samples before and after 
outdoor incubation in the presence and absence of UV-B and UV-A 
radiation. Accompanying measurements of detrital-corrected 
absorption spectra (with the aid of an integrating sphere), 
fluorescence excitation spectra, and fluorescence decay kinetics
(with  the aid of a Pulse-Amplitude-Fluorimeter [PAM]) have been 
successful and a now a part of routine measurements to define the 
optical biology and photosensitivity of natural phytoplankton 
communities to UV radiation. We also completed the field 
measurement of a biological weighting function for UV-inhibition of 
primary production in Antarctic phytoplankton.


S-014     Energetics of the Adults and Larvae of the Antarctic
          Krill, Euphausia superba.  Principal Investigators:  L.B.
          Quetin and R.M Ross. University of California at Santa
          Barbara.

          Field Team:  T. Frazer, C. Wyatt and J. Mahoney

     Larval krill continue to be reared under varying food and
temperature regimes.  Subsamples of larvae from each experimental
treatment are removed periodically and prepared for analysis of
stable isotopic, elemental and chemical composition.  Respiration
rates of animals in each of the experimental treatments are
measured prior to preparation procedures and information on growth
rate and molting frequency is also recorded.  Data are being
organized and formatted for comparison with field collected data
from June (WINCRUISE V) and other previous field seasons.  Post-
cruise (WINCRUISE V) processing of krill samples continues, e.g.
determination of lipid and protein content.

     Field team member C. Wyatt (S-014/S-028) prepared and staged
supplies in anticipation of the August/September LTER cruise and on
September 29, T. Frazer departed Palmer Station as a participant on
the LTER cruise.  Ongoing laboratory experiments will be carried
out by field team members remaining on station.
   
  
S-032     Long-Term Ecological Research on the Marine
          Ecosystem:  An Ice-Dominated System.  Principal
          Investigator:  R.C. Smith.

and

S-034     Ozone dimunition, Ultraviolet Radiation and Phytoplankton
          Biology in Antarctic Waters.  Co-Principal Investigators
          R.C. Smith (S-034) and B. Prezelin (S-010).  University
          of California at Santa Barbara.

          Field Team:  R. Smith, D. Menzies, C. Johnson, P.
          Handley, E. Bruce and E. Fields 

     Arrived on station 12 August.  Set up labs and checked out
equipment:
     
     BOPS2 -  This instrument was used on the March/April cruise,
     and since there was no time to return it to Santa Barbara, it 
     had been stored in PA warehouse.  The BOPS2 was checked out
     new software tested, and a recalibration will be done between
     the LTER and Ice93 cruises.

     Computer Networking - for various field computers and 
     instruments was set up and tested.

     PUV500 & PUV510 - These instruments were tested, and then
     set up at T-5 for intercomparison with the BSI UV monitoring
     system.

     ROZE - The zodiac operation for CTD, fluorescence,
     transmittance and the OFFI were set up and checked out in the
     boat house.

     LUVSS - This instrument was set up and tested.  Our intent is
     to use the deck-LUVSS set up at T-5 for an intercomparison
     with the BSI UV monitoring system.
     
     Turner Fluorometers - Calibration and intercalibration of
     several turner fluorometers and the HPLC system used by
     Prezelin's group was carried out.

     Ice conditions prevented starting a regular LTER sampling
effort within the Palmer grid although samples for chl were
obtained in collaboration with Prezelin's group.  In addition, and
under ice dive was carried out in Hero Inlet and the PUV instrument
were deployed to acertain the sensitivity of this instrument for
measuring the penetration of UV through inc.


S-106     Stanford VLF. U. Inan, Stanford University.

     Daily data continues to be collected and prepared for
retrograde.  There was a power outage on August 12, 1993.  Digital
data collection was interrupted and GEOS satellite lock was lost
for several days.  System was brought back on line and GEOS was
later re-acquired.  System being operated by ASA science
technicians Andrew Archer and John Booth.


S-275     UM/DOE Atmospheric Monitoring Program at Palmer Station.
          T. Snowdon, University of Miami; C. Sanderson/N. Chui,
          EML/DOE N.Y.

     No personnel on station. System being run by ASA science
technician.  System continues to operate on a normal weekly
schedule of calibration, background, and sample counts, with one
sample filter being exposed for the duration of the week.


T-312     Terascan satellite imaging system. R. Whritner, Scripps
          Institute of Oceanography.

     PI on station.  Installation of SPARC-2 successful; system now
schedules, collects, and archives data from multiple satellites
automatically.  Telemetry data is being processed and examined
daily, and ice images are being produced and sent to ATSVAX.


T-313     UV Monitoring Experiment. C. Booth, Biospherical
          Instruments.

     No personnel on station.  System is being operated by station
science technician.  The system is successfully collecting UV data. 
There was a power outage on August 12, 1993, but the system was
brought back on line immediately.  An absolute calibration
intercomparison was performed on August 4, 1993, and an absolute
calibration with the site standard was performed on August 20,
1993.  High voltages were dropped on data and response scans due to
brightening conditions.


S-091     Seismic Observatory, United States Geological Survey.

     No personnel on station, system is being monitored by station
science technician.  Data is successfully collected and prepared
for retrograde.  There was a power outage on August 12, 1993;  DP
computer reset and changed tapes automatically; DA computer was
unaffected.

T. Frazer/PEOPLEAN

 
03230820.764
PLM215.SEP
