pigmhplc 9900PAL 11-01-99 through 03-19-00 Samples taken from Go-Flo bottles and analyzed with HPLC for photosynthetic pigments. year, julian day, date, station, time (GMT hour), time (GMT minutes), latitude (degrees), lat. (minutes), longitude (degrees), long. (minutes), irradiance (%), depth (meters), chlorophyll c3 (ug/l), chlorophyll c2 (ug/l), peridinin (mg/l), 19'-butanoyloxyfucoxanthin (ug/l), fucoxanthin (ug/l), prasinoxanthin (mg/l), 19'-hexanoyloxyfucoxanthin (ug/l), diadinoxanthin (ug/l), alloxanthin (ug/l), diatoxanthin (ug/l), zeaxanthin (ug/l), lutein (ug/l), chlorophyll b (ug/l), chlorophyll a (ug/l), alpha carotene (ug/l), beta carotene (ug/l) Water was sampled at Palmer inshore stations B and E twice weekly. Samples were collected using General Oceanics Inc. Go-Flo bottles deployed off a zodiac, tripped at the surface and at depths corresponding to 60, 30, 10, 5, and 1 percent of measured surface PAR. Samples were filtered onto GF/F filters, frozen in liquid nitrogen and transported to SIO in San Diego where they were analyzed within ten months of collection. At SIO samples were extracted in 90% acetone for 24 hours at -60 degrees Celsius. Samples were then injected onto a Waters Symmetry C8 150mm column and separated by HPLC using a method adapted from Zapata et al, 2000. Peaks were identified based on retention times of standards from DHI Water & Environment and integrated using Water's Millineum 32 system; concentrations were calculated based on external standard curves in Microsoft Excel. Water sampled from Go-Flo bottles, which corresponded to light levels used in primary productivity simulated in situ experiments (see Sampling Frequency, above). HPLC, pigments ASCII, comma separated 9900pal/pigmhplc 9900pal.pigmhplc Dr. Maria Vernet, Scripps Institution of Oceanography, San Diego, CA mvernet@ucsd.edu Dr. Maria Vernet, Scripps Institution of Oceanography, San Diego, CA mvernet@ucsd.edu Karie Sines Jill Glass Meghan Duffy Maria Vernet Wendy Kozlowski Andres Hall Karie Sines Maria Vernet Andres Hall Karie Sines Karie Sines 12-30-02 09-02-03, 09-24-03 Zapata, M., F. Rodriguez and J.L. Garrido. Separation of chlorophylls and carotenoids from marine phytoplankton: a new HPLC method using a reversed phase C8 column and pyridine-containing mobile phases. Marine Ecology Progress Series. March 2000 V195 p.29-45. Values changed due to corrections made in formulas for calculation of concentrations. -999 represents data not available. SWI represents sampling from the seawater intake at Palmer Station. Concentrations based on calibrations done at SIO from November 2000 through February 2001. Please note that this data set may be updated as adjustments are made to instrument calibration.