HPLC 98JANB 01-28-98 through 02-12-98 Photosynthetic pigments of watercolumn samples using high performance liquid chromatography year, decimal day, date, latitude (decimal degrees), longitude (decimal degrees), LTER grid line, LTER grid station, irradiance (%), LTER event #, depth (m), chlorophyll c2 (ug/l), peridinin (ug/l), 19'-butanoyloxyfucoxanthin (ug/l), fucoxanthin (ug/l), 19'-hexanoyloxyfucoxanthin (ug/l), diadinoxanthin (ug/l), alloxanthin (ug/l), diatoxanthin (ug/l), lutein (ug/l), zeaxanthin (ug/l), chlorophyll b (ug/l), chlorophyll a (ug/l), alpha carotene (ug/l), beta carotene (ug/l) Water was sampled at all stations with the exception of the Palmer inshore stations F -J, from Go-Flo bottles tripped at the surface, and bottles tripped at depths corresponding to 50, 25, 10, 5, and 1 percent of measured surface PAR. Samples were filtered onto GF/F filters and frozen for a minimum of 24 hours. Pigments were then extracted in 90% acetone for 24 hours at -60 degrees Celsius. Samples were then injected onto a Waters C18 300mm column and separated by HPLC using a method adapted from Wright et al, 1991. Peaks were identified by diode array spectrum analysis and integrated using Hitachi's D-7000 system; concentrations were calculated based on external standard curves in Microsoft Excel. Water sampled from Go-Flo bottles, which corresponded to light levels used in primary productivity simulated in situ experiments (see Sampling Frequency, above). HPLC, pigments ASCII, comma separated 98janb/pigmhplc 98janb.pigmhplc Maria Vernet Dr. Maria Vernet, Scripps Institution of Oceanography, San Diego, CA mvernet@ucsd.edu Wendy Kozlowski, Karie Sines, Michael Crowder, Marnie Zirbel Karie Sines, Wendy Kozlowski Wendy Kozlowski, Karie Sines Wendy Kozlowski Wendy Kozlowski 05-30-00 Wright, S.W., S.W. Jeffrey, R.F.C. Mantoura, C.A. Llewellyn, T. Bjorland, D. Repeta and N. Welschmeyer. Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Marine Ecology Progress Series. Nov. 1991 V77 n2-3 p.183-196. -999 represents data not available. Approximately 30% of the samples collected were thawed and lost in shipment to CONUS for analysis. Concentration based on calibrations done in the 1996-1997 season. Please note that this data set may be updated as adjustments are made to instrument calibration.