pigmhplc 9899PAL 10-13-98 through 01-23-99 Samples taken from Go-Flo bottles and analyzed with HPLC for photosynthetic pigments. year, decimal day, date, latitude (decimal degrees) longitude (decimal degrees) station, irradiance (%), depth (m), chlorophyll c2 (ug/l), peridinin (ug/l), 19'-butanoyloxyfucoxanthin (ug/l), fucoxanthin (ug/l), 19'-hexanoyloxyfucoxanthin (ug/l), diadinoxanthin (ug/l), alloxanthin (ug/l), diatoxanthin (ug/l), lutein (ug/l), zeaxanthin (ug/l), chlorophyll b (ug/l), chlorophyll a (ug/l), alpha carotene (ug/l), beta carotene (ug/l) Water was sampled at Palmer inshore stations B and E twice weekly. Samples were collected using General Oceanics Inc. Go-Flo bottles deployed off a zodiac, tripped at the surface and at depths corresponding to 50, 25, 10, 5, and 1 percent of measured surface PAR. Samples were filtered onto GF/F filters and frozen at -60 degrees Celsius for a minimum of 24 hours. Pigments were extracted in 90% acetone for 24 hours at -60 degrees Celsius. Samples were then injected onto a Waters C18 300mm column and separated by HPLC using a method adapted from Wright and Jeffrey, 1991. Peaks were identified by diode array spectrum analysis and integrated using Hitachi's D-7000 system; concentrations were calculated based on external standard curves in Microsoft Excel. Water sampled from Go-Flo bottles, which corresponded to light levels used in primary productivity simulated in situ experiments (see Sampling Frequency, above). HPLC, pigments ASCII, comma separated 9899pal/pigmhplc 9899pal.pigmhplc Dr. Maria Vernet, Scripps Institution of Oceanography, San Diego, CA mvernet@ucsd.edu Dr. Maria Vernet, Scripps Institution of Oceanography, San Diego, CA mvernet@ucsd.edu Wendy Kozlowski, Jennifer White, Eric Pohlman, Karen Weinbaum Wendy Kozlowski Wendy Kozlowski, Karen Pelletreau, Michael Thimgan Wendy Kozlowski Wendy Kozlowski 06-18-01 Wright, S.W., S.W. Jeffrey, R.F.C. Mantoura, C.A. Llewellyn, T. Bjorland, D. Repeta and N. Welschmeyer. Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Marine Ecology Progress Series. Nov. 1991 V77 n2-3 p.183-196. -999 represents data not available. Concentrations based on calibrations done in the 1998-1999 field season, with the exception of lutein, zeaxanthin and beta carotene, which are based on 1997 calibrations. Please note that this data set may be updated as adjustments are made to instrument calibration.