HPLC 97JAN 11-08-97 through 02-12-97 Photosynthetic pigments of watercolumn samples using high performance liquid chromatography year, julian day, date, GMT (hour), GMT (minute), latitude (degrees), latitude (minutes), longitude (degrees), longitude (minutes), LTER grid line (#), station/location description, irradiance (%), LTER event #, depth (m), chlorophyll c2 (ug/l), peridinin (ug/l), 19'-butanoyloxyfucoxanthin (ug/l), fucoxanthin (ug/l), 19'-hexanoyloxyfucoxanthin (ug/l), diadinoxanthin (ug/l), alloxanthin (ug/l), diatoxanthin (ug/l), lutein (ug/l), zeaxanthin (ug/l), chlorophyll b (ug/l), chlorophyll a (ug/l), alpha carotene (ug/l), beta carotene (ug/l) Water was sampled at all stations with the exception of the Palmer inshore stations F -J, from BOPS bottles tripped at the surface (bottle #11), and bottles tripped at depths corresponding to 50, 30, 13, 4, and 0.5 percent of measured surface PAR (usually bottles 10-6). Samples taken from BOPS water profiling system Nisken bottles and analyzed with HPLC for pigments. Samples were filtered onto GF/F filters and frozen for a minimum of 24 hours. Pigments were then extracted in 90% acetone for 24 hours at -60 degrees Celsius. Samples were then injected onto a Waters C18 300mm column and separated by HPLC using a method adapted from Wright et al, 1991. Peaks were identified by diode array spectrum analysis and integrated using Hitachi's D-7000 system; concentrations were calculated based on external standard curves in Microsoft Excel. Water sampled from BOPS bottles, which corresponded to light levels used in primary productivity simulated in situ experiments (see Sampling Frequency, above). HPLC, pigments ASCII, comma separated 97jan/pigmhplc 97jan.pigmhplc Maria Vernet Maria Vernet, Scripps Institution of Oceanography, San Diego, CA mvernet@ucsd.edu Wendy Kozlowski Karie Sines Joe Fontaine Cristine Braun Moraes Danielle Harvey Wendy Kozlowski Wendy Kozlowski Wendy Kozlowski Wendy Kozlowski 05-21-99 Wright, S.W., S.W. Jeffrey, R.F.C. Mantoura, C.A. Llewellyn, T. Bjorland, D. Repeta and N. Welschmeyer. Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Marine Ecology Progress Series. Nov. 1991 V77 n2-3 p.183-196. -999 represents data not available.