pigmhplc 9798PAL 12-15-97 through 03-24-98 Samples taken from Go-Flo bottles and analyzed with HPLC for photosynthetic pigments. year, decimal day, date, latitude (decimal degrees) longitude (decimal degrees) station, irradiance (%), depth (m), chlorophyll c2 (ug/l), peridinin (ug/l), 19'-butanoyloxyfucoxanthin (ug/l), fucoxanthin (ug/l), 19'-hexanoyloxyfucoxanthin (ug/l), diadinoxanthin (ug/l), alloxanthin (ug/l), diatoxanthin (ug/l), lutein (ug/l), zeaxanthin (ug/l), chlorophyll b (ug/l), chlorophyll a (ug/l), alpha carotene (ug/l), beta carotene (ug/l) Water was sampled at Palmer inshore stations B and E twice weekly. Samples were collected using General Oceanics Inc. Go-Flo bottles deployed off a zodiac, tripped at the surface and at depths corresponding to 50, 25, 10, 5, and 1 percent of measured surface PAR. Samples were filtered onto GF/F filters and frozen at -60 degrees Celsius for a minimum of 24 hours. Pigments were extracted in 90% acetone for 24 hours at -60 degrees Celsius. Samples were then injected onto a Waters C18 300mm column and separated by HPLC using a method adapted from Wright and Jeffrey, 1991. Peaks were identified by diode array spectrum analysis and integrated using Hitachi's D-7000 system; concentrations were calculated based on external standard curves in Microsoft Excel. Water sampled from Go-Flo bottles, which corresponded to light levels used in primary productivity simulated in situ experiments (see Sampling Frequency, above). HPLC, pigments ASCII, comma separated 9798pal/pigmhplc 9798pal.pigmhplc Maria Vernet Dr. Maria Vernet, Scripps Institution of Oceanography, San Diego, CA mvernet@ucsd.edu Wendy Kozlowski, Karie Sines, Michael Crowder, Marnie Zirbel Karie Sines, Wendy Kozlowski Wendy Kozlowski, Karie Sines Wendy Kozlowski Wendy Kozlowski 05-30-00 Wright, S.W., S.W. Jeffrey, R.F.C. Mantoura, C.A. Llewellyn, T. Bjorland, D. Repeta and N. Welschmeyer. Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Marine Ecology Progress Series. Nov. 1991 V77 n2-3 p.183-196. -999 represents data not available. Approximately 30% of the samples collected this season were thawed and lost in shipment to CONUS for analysis. Concentration based on calibrations done in the 1996-1997 season. Please note that this data set may be updated as adjustments are made to instrument calibration.