pigmhplc 9697PAL 12-08-96 through 03-18-97 Photosynthetic pigments of watercolumn samples using high performance liquid chromatography year, julian day, date, station, GMT(hour), GMT(min.), latitude (deg.), latitude (min.), longitude (deg.), longitude (min.), irradiance (%), depth (m), chlorophyll c2 (ug/l), peridinin (ug/l), 19'-butanoyloxyfucoxanthin (ug/l), fucoxanthin (ug/l), 19'-hexanoyloxyfucoxanthin (ug/l), diadinoxanthin (ug/l), alloxanthin (ug/l), diatoxanthin (ug/l), lutein (ug/l), zeaxanthin (ug/l), chlorophyll b (ug/l), chlorophyll a (ug/l), alpha carotene (ug/l), beta carotene (ug/l) Water was sampled at Palmer inshore stations B and E twice weekly. Samples were collected using General Oceanics Inc. Nisken bottles deployed off a zodiac, tripped at the surface and at depths corresponding to 50, 30, 13, 4, and 0.5 percent of measured surface PAR. Samples were filtered onto GF/F filters and frozen at -60 degrees Celsius for a minimum of 24 hours. Pigments were then extracted in 90% acetone for 24 hours at -60 degrees Celsius. Samples were then injected onto a Waters C18 300mm column and separated by HPLC using a method adapted from Wright and Jeffrey, 1991. Peaks were identified by diode array spectrum analysis and integrated using Hitachi's D-7000 system; concentrations were calculated based on external standard curves in Microsoft Excel. Water sampled from Nisken bottles, which corresponded to light levels used in primary productivity simulated in situ experiments (see Sampling Frequency, above). HPLC, pigments ASCII, comma separated 9697pal/pigmhplc 9697pal.pigmhplc Maria Vernet Dr. Maria Vernet, Scripps Institution of Oceanography, San Diego, CA mvernet@ucsd.edu Wendy Kozlowski Karie Sines Joe Fontaine Jeffrey Walker Eric Holm Wendy Kozlowski Wendy Kozlowski Jennifer White Wendy Kozlowski Wendy Kozlowski 05-21-99 Wright, S.W., S.W. Jeffrey, R.F.C. Mantoura, C.A. Llewellyn, T. Bjorland, D. Repeta and N. Welschmeyer. Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Marine Ecology Progress Series. Nov. 1991 V77 n2-3 p.183-196. -999 represents data not available. Concentration based on calibrations done in the 1996-1997 season. Please note that this data set is not final - changes may be made with finalization of calibration.