pigmhplc 9495pal 22nov94-27feb95 Photosynthetic pigments of watercolumn samples using high performance liquid chromatography julian year, julian day, lat. (deg.), lat. (min.), lat (sec), long. (deg.), long. (min.), long (sec.), time(hour), time(min.), LTER event #, station, irradiance (%), depth (m), chlorophyll c2 (ug/l), peridinin (ug/l), 19'-butanoyloxyfucoxanthin (ug/l), fucoxanthin (ug/l), 19'-hexanoyloxyfucoxanthin (ug/l), diadinoxanthin (ug/l), alloxanthin (ug/l), diatoxanthin (ug/l), lutein (ug/l), zeaxanthin (ug/l), chlorophyll b (ug/l), chlorophyll a (ug/l), alpha carotene (ug/l), beta carotene (ug/l) Water was sampled at Palmer inshore stations B and E twice weekly and at Palmer inshore stations C and D once weekly. Samples were collected using a Go-Flo bottle deployed off a zodiac, tripped at the surface and at depths corresponding to 55, 27, 11, 5, and 2 percent of measured PAR. There were two timeframes of this work: 22nov94-02jan95 and 13feb95-27feb95 Samples taken from Go-Flo bottles and analyzed with HPLC for photosynthetic pigments. Samples were filtered onto GF/F filters and frozen at -60 degrees Celcius for a minimum of 24 hours. Pigments were then extracted in 90% acetone for 24 hours at -60 degrees Celsius. Samples were then injected onto a Waters C18 300mm column and separated by HPLC using a method adapted from Wright and Jeffrey, 1991. Peaks were identified by diode array spectrum analysis and integrated using Hitachi's D-6500 system; concentrations were calculated based on external standard curves in Microsoft Excel. Water sampled from Go-Flo bottles which corresponded to light levels used in primary productivity simulated in situ experiments (see Sampling Frequency, above). lter, antarctic, HPLC, pigments ASCII, comma separated 9495pal.hplc Maria Vernet Wendy Kozlowski Maria Vernet Wendy Kozlowski, Ted Ruel, John Harrison, Karen Patterson, Douglas Ikelheimer Wendy Kozlowski Wendy Kozlowski Wendy Kozlowski Wendy Kozlowski 12-31-96 09-25-97 Wright, S.W., S.W. Jeffrey, R.F.C. Mantoura, C.A. Llewellyn, T. Bjorland, D. Repeta and N. Welschmeyer. Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Marine Ecology Progress Series. Nov. 1991 V77 n2-3 p.183-196. -999 represents data not available. Concentration based on calibrations done in the 1995-1996 season. Please note that this data set is not final - changes may be made with finalization of calibration.