HPLC 01JAN 10 Jan 01 through 24 Jan 01 Samples taken from rosette Go-Flo bottles and analyzed with HPLC for pigments. year, julian day, date, latitude (decimal degrees), longitude (decimal degrees), LTER grid line, LTER grid station, irradiance (%), LTER event #, depth (m), chlorophyll c2 (ug/l), 19'-butanoyloxyfucoxanthin (ug/l), fucoxanthin (ug/l), 19'-hexanoyloxyfucoxanthin (ug/l), diadinoxanthin (ug/l), diatoxanthin (ug/l), zeaxanthin (ug/l), lutein (ug/l), chlorophyll b (ug/l), chlorophyll a (ug/l), alpha carotene (ug/l) Water was sampled at all stations with the exception of the Palmer inshore stations F -J, from Go-Flo bottles tripped at the surface, and bottles tripped at depths corresponding to 50, 25, 10, 5, and 1 percent of measured surface PAR. Samples were filtered onto GF/F filters and frozen in liquid nitrogen for a minimum of 24 hours. Filters were extracted in 90% acetone for 24 hours at -60 degrees Celsius. Samples were then injected onto a C8 150mm column and separated by HPLC using a method adapted from Zapata et al, 2000. Peaks were identified by diode array spectrum analysis and by retention times of standards obtained from DHI Water & Environment and integrated using Hewlett Packard's 1100 Series system; concentrations were calculated based on external standard curves in Microsoft Excel. Water sampled from Go-Flo bottles, which corresponded to light levels used in primary productivity simulated in situ experiments (see Sampling Frequency, above). HPLC, pigments ASCII, comma separated 01jan/pigmhplc 01jan.pigmhplc Dr. Maria Vernet, Scripps Institution of Oceanography, San Diego, CA mvernet@ucsd.edu Maria Vernet, Scripps Institution of Oceanography, San Diego, CA mvernet@ucsd.edu Wendy Kozlowski Erin Macri Silvia Rodriguez Wendy Kozlowski Erin Macri Karie Sines Wendy Kozlowski Karie Sines Karie Sines 02 Sept 03 Zapata, M., F. Rodriguez and J.L. Garrido. Separation of chlorophylls and carotenoids from marine phytoplankton: a new HPLC method using a reversed phase C8 column and pyridine-containing mobile phases. Marine Ecology Progress Series. March 2000 V195 p.29-45. -999 represents data not available. Concentrations based on calibrations done at Palmer Station from November 2000 through February 2001.