HPLC 00JAN 01-09-00 through 01-26-00 Samples taken from rosette Go-Flo bottles and analyzed with HPLC for pigments. year, julian day, date, latitude (decimal degrees), longitude (decimal degrees), LTER grid line, LTER grid station, irradiance (%), LTER event #, depth (m), chlorophyll c3 (ug/l), chlorophyll c2 (ug/l), peridinin (ug/l), 19'-butanoyloxyfucoxanthin (ug/l), fucoxanthin (ug/l), prasinoxanthin (ug/l), 19'-hexanoyloxyfucoxanthin (ug/l), diadinoxanthin (ug/l), alloxanthin (ug/l), diatoxanthin (ug/l), zeaxanthin (ug/l), lutein (ug/l), chlorophyll b (ug/l), chlorophyll a (ug/l), alpha carotene (ug/l), beta carotene (ug/l) Water was sampled at all stations with the exception of the Palmer inshore stations F -J, from Go-Flo bottles tripped at the surface, and bottles tripped at depths corresponding to 50, 25, 10, 5, and 1 percent of measured surface PAR. Samples were filtered onto GF/F filters, frozen in liquid nitrogen and transported to SIO in San Diego where they were analyzed within ten months of collection. At SIO samples were extracted in 90% acetone for 24 hours at -60 degrees Celsius. Samples were then injected onto a Waters Symmetry C8 150mm column and separated by HPLC using a method adapted from Zapata et al, 2000. Peaks were identified based on retention times of standards obtained from DHI Water & Environment and integrated using Water's Millineum 32 system; concentrations were calculated based on external standard curves in Microsoft Excel. Water sampled from Go-Flo bottles, which corresponded to light levels used in primary productivity simulated in situ experiments (see Sampling Frequency, above). HPLC, pigments ASCII, comma separated 00jan/pigmhplc 00jan.pigmhplc Dr. Maria Vernet, Scripps Institution of Oceanography, San Diego, CA mvernet@ucsd.edu Maria Vernet, Scripps Institution of Oceanography, San Diego, CA mvernet@ucsd.edu Maria Vernet Wendy Kozlowski Karie Sines Meghan Duffy Jill Glass Veronica Vigilant Maria Vernet Wendy Kozlowski Andres Hall Karie Sines Maria Vernet Andres Hall Karie Sines Karie Sines 12-30-02 09-02-03,09-24-03 Zapata, M., F. Rodriguez and J.L. Garrido. Separation of chlorophylls and carotenoids from marine phytoplankton: a new HPLC method using a reversed phase C8 column and pyridine-containing mobile phases. Marine Ecology Progress Series. March 2000 V195 p.29-45. Values changed due to corrections in formulas for calculating concentrations. -999 represents data not available. Concentrations based on calibrations done at SIO from November 2000 through February 2001. Please note that this data set may be updated as adjustments are made to instrument calibration.