pigmhplc 0001PAL 10-13-00 through 03-29-01 Samples taken from Go-Flo bottles and analyzed with HPLC for photosynthetic pigments. year, julian day, date, station, time (GMT hour), time (GMT minutes), latitude (degrees), lat. (minutes), longitude (degrees), long. (minutes), irradiance (%), depth (meters), chlorophyll c2 (ug/l), 19'-butanoyloxyfucoxanthin (ug/l), fucoxanthin (ug/l), 19'-hexanoyloxyfucoxanthin (ug/l), diadinoxanthin (ug/l), diatoxanthin (ug/l), zeaxanthin (ug/l), lutein (ug/l), chlorophyll b (ug/l), chlorophyll a (ug/l), alpha carotene (ug/l) Water was sampled at Palmer inshore stations B and E twice weekly. Samples were collected using General Oceanics Inc. Go-Flo bottles deployed off a zodiac, tripped at the surface and at depths corresponding to 60, 30, 10, 5, and 1 percent of measured surface PAR. Samples were filtered onto GF/F filters, frozen in liquid nitrogen and analyzed within three months of collection. Filters were extracted in 90% acetone for 24 hours at -60 degrees Celsius. Samples were then injected onto a Waters Symmetry C8 150mm column and separated by HPLC using a method adapted from Zapata et al, 2000. Peaks were identified based on diode array spectrum and by retention times of standards from DHI Water & Environment and integrated using Hewlett Packard's 1100 Series system; concentrations were calculated based on external standard curves in Microsoft Excel. Water sampled from Go-Flo bottles, which corresponded to light levels used in primary productivity simulated in situ experiments (see Sampling Frequency, above). HPLC, pigments ASCII, comma separated 0001pal/pigmhplc 0001pal.pigmhplc Dr. Maria Vernet, Scripps Institution of Oceanography, San Diego, CA mvernet@ucsd.edu Dr. Maria Vernet, Scripps Institution of Oceanography, San Diego, CA mvernet@ucsd.edu Wendy Kozlowski Karie Sines Silvia Rodriguez Jeff Bechtel Karen Pelletreau Micheal Thimgan Wendy Kozlowski Karie Sines Karie Sines Wendy Kozlowski Karie Sines Karie Sines 09-02-03 Zapata, M., F. Rodriguez and J.L. Garrido. Separation of chlorophylls and carotenoids from marine phytoplankton: a new HPLC method using a reversed phase C8 column and pyridine-containing mobile phases. Marine Ecology Progress Series. March 2000 V195 p.29-45. -999 represents data not available. Concentrations based on calibrations done at Palmer Station from November 2000 through February 2001.